產品目錄
Caspase-9活性測定試劑盒 BC3890
物品單位 價格 品牌
780 Solarbio
  • 產地:北京
  • 貨號:BC3890
  • 發布日期: 2019-12-16
  • 更新日期: 2019-12-16
產品詳細說明
產地 北京
品牌 Solarbio
貨號 BC3890
用途 Caspase-9活性測定
產品規格 20T
CAS編號
純度 現詢客服%
是否進口

Caspase-9也稱ICE-LAP6或Mch6,可與細胞色素c和Apaf1形成復合物被激活,并進一步激活細胞凋亡的最關鍵酶caspase-3,從而觸發凋亡級聯反應。因此,Caspase-9是細胞凋亡信號轉導過程中重要的上游caspase。
細胞凋亡屬于程序化細胞死亡,可見于各種器官和細胞,在正常發育、生理、病理過程中對細胞和器官的穩態具有十分重要的調節作用。Caspase (Cysteine-requiring Aspartate Protease)是參與細胞凋亡過程的蛋白酶家族,包含10多個成員。Caspase-9也稱ICE-LAP6或Mch6,可與細胞色素c和Apaf1形成復合物被激活,并進一步激活細胞凋亡的最關鍵酶caspase-3,從而觸發凋亡級聯反應。因此,Caspase-9是細胞凋亡信號轉導過程中重要的上游caspase。其激活可以通過磷酸化進行調控。試劑盒測定原理基于Caspase-9特異水解其多肽底物Ac-LEHD-pNA (N-acetyl-Leu-Glu-His-Asp-p-nitroanilide),釋放出游離的硝基苯胺pNA,后者呈黃色在405 nm具有*吸收峰,用可見光分光光度比色方法測定。其吸光度值對應于Caspase-9的水解活性。反應體系100微升,試劑盒提供的試劑,除標準曲線外可進行50或100次樣品測定。適用:測定哺乳動物組織、細胞caspase-9活性。

組成與儲存:
1. 裂解緩沖液: 20 ml ,4 oC
2. 反應緩沖液: 10 ml ,4 oC
3. 2 mM Ac-LEHD-pNA底物: 0.5 ml,-20 oC避光
4. 10 mM pNA標準品: 0.5 ml,-20 oC避光
以上標出的量為100次檢測,50次檢測的試劑量減半。試劑常溫運輸。到達后按要求儲存,1年內穩定。

所需儀器:
酶標儀與96孔酶標板;或可見光分光光度計配100μl容積的石英比色杯。
工作波長:*吸收波長405 nm,如不具備也可在400~450 nm范圍內測定。

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