產品目錄
4%組織細胞固定液 P1110
物品單位 價格 品牌
60 solarbio
  • 產地:北京
  • 貨號:P1110
  • 發布日期: 2020-03-31
  • 更新日期: 2020-03-31
產品詳細說明
產地 北京
品牌 solarbio
貨號 P1110
用途范圍 是免疫組織化學(IHC)和免疫細胞化學(ICC)研究中最常用的固定劑之一。
英文名稱 Paraformaldehyde,4%
純度 100ml%
CAS編號
規格 100ml
保質期 -20℃,1年
是否進口
英文名稱Paraformaldehyde,4%
儲存條件-20℃,有效期1年
單位

產品說明:

多聚甲醛(Paraformaldehyde)是免疫組織化學(IHC)和免疫細胞化學(ICC)研究中最常用的固定劑之一。該固定劑較為溫和,能很好地保存組織的抗原性和細微結構,適于組織標本和細胞片的較長期保存,非常適合組織和細胞的光鏡免疫化學研究。

本產品為4%多聚甲醛的PBS溶液,可直接用于組織和細胞固定。若需使用其他低濃度多聚甲醛,可使用0.01M PBS按比例稀釋。

使用說明:

組織固定:室溫或4℃浸泡固定2-24h,用緩沖液漂洗后即可樹脂包埋用于組織切片。

切片固定:室溫或4℃固定10min-2h,視切片厚度而定,用緩沖液漂洗后即可用于染色。

細胞固定:室溫或4℃固定10-30min,用緩沖液漂洗后即可用于染色。

注意事項:

為了您的安全和健康,請穿實驗服并戴一次性手套操作。

本產品不含防腐劑,不宜室溫或4℃長期保存,請勿反復凍融。



參考文獻:


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《Collagen facilitates the colorectal cancer stemness and metastasis through an integrin/PI3K/AKT/Snail signaling pathway》 作者:Xiangbin Wu,Jianhui Cai,Zhigui Zuo,Jinlei Li 期刊:Biomedicine & Pharmacotherapy 影響因子:3.743 PMID:30913493

《Proteomic Analyses of Mammary Glands Provide Insight into the Immunity and Metabolism Pathways Associated with Clinical Mastitis in Meat Sheep》 作者:Jianfeng Gao, Taotao Li, Zengkui Lu, Xia Wang, Xingxu Zhao  and Youji Ma 期刊:Animals 影響因子:1.832 PMID:31159303

《The treatment of systematically transplanted gingival mesenchymal stem cells in periodontitis in mice》 作者:Wendong Sun, Zhiguo Wang, Quanchen Xu, Huibin Sun, Xiaoxuan Liu, Jianjun Yang, Rundan Hong 期刊:Experimental and Therapeutic Medicine 影響因子:1.448 PMID:30783482

《MMP-9 secreted by tumor associated macrophages promoted gastric cancer metastasis through a PI3K/AKT/Snail pathway》 作者:Liu Lin,Yu Ye,Xiumei Zhu 期刊:Biomedicine & Pharmacotherapy 影響因子:3.743 PMID:31202170

《Macropinocytosis-dependent endocytosis of Japanese flounder IgM+ B cells and its regulation by CD22》 作者:Yiqun Li,Li Sun,Jun Li 期刊:Fish & Shellfish Immunology 影響因子:3.298 PMID:30266605

《Escin suppresses HMGB1‐induced overexpression of aquaporin‐1 and increased permeability in endothelial cells》 作者:Changjun Chen, Songgang Wang, Jiying Chen, Xiaolin Liu, Mengchen Zhang, Xi Wang, Weihua Xu,Yayun Zhang, Hao Li, Xin Pan, Meng Si 期刊:FEBS Open Bio homepage 影響因子:1.959 PMID:30972964

《Salvanic acid B inhibits myocardial fibrosis through regulating TGF-β1/Smad signaling pathway》 作者:Hongyan Gao,Zhe Bo,Qin Wang,Ling Luo,Haiyi Zhu,Yi Ren 期刊:Biomedicine & Pharmacotherapy 影響因子:3.743 PMID:30553195

《Long non‐coding RNA MALAT1 regulates angiogenesis following oxygen‐glucose deprivation/reoxygenation》 作者:Chengya Wang, Youyang Qu, Rui Suo, Yulan Zhu 期刊:Journal of Cellular and Molecular Medicine 影響因子:4.658 PMID:30784209

《Identification of oxidative stress–related Xdh gene as a di(2‐ethylhexyl)phthalate (DEHP) target and the use of melatonin to alleviate the DEHP‐induced impairments in newborn mouse ovaries》 作者:Jing‐Cai Liu, Lan Li, Hong‐Chen Yan, Teng Zhang, Pan Zhang, Zhong‐Yi Sun, Massimo De Felici, Russel J. Reiter, Wei Shen 期刊:Journal of Pineal Research 影響因子:15.221 PMID:30938853

《Toxoplasma gondii ROP17 inhibits the innate immune response of HEK293T cells to promote its survival》 作者:Jie-Xi Li,Jun-Jun He,Hany M. Elsheikha,Dan Chen,Bin-Tao Zhai,Xing-Quan Zhu,Hai-Kuo Yan 期刊:Parasitology Research 影響因子:2.067 PMID:30675671

《The role of N6-methyladenosine RNA methylation in the heat stress response of sheep (Ovis aries)》 作者:Zengkui Lu,Youji Ma,Qing Li,Enmin Liu,Meilin Jin,Liping Zhang,Caihong Wei 期刊:Cell Stress and Chaperones 影響因子:2.903 PMID:30701478

《iTRAQ‐Based Proteomic Analysis Exploring the Influence of Hypoxia on the Proteome of Dental Pulp Stem Cells under 3D Culture》 作者:Lei Dou, Qifang Yan, Panpan Liang, Pengfei Zhou, Yan Zhang, Ping Ji 期刊:Proteomics 影響因子:3.106 PMID:29327447

《Dentinogenesis and Tooth-Alveolar Bone Complex Defects in BMP9/GDF2 Knockout Mice》 作者:Xia Huang, Feilong Wang, Chen Zhao, Sheng Yang, Qianyu Cheng, Yingying Tang, Fugui Zhang, Yan Zhang, Wenping Luo, Chao Wang, Pengfei Zhou, Stephanie Kim, Guowei Zuo, Ning Hu, Ruidong Li, Tong-chuan He 期刊:Stem Cells and Development 影響因子:3.147 PMID:30816068

《Epigenetically upregulated oncoprotein PLCE1 drives esophageal carcinoma angiogenesis and proliferation via activating the PI-PLCε-NF-κB signaling pathway and VEGF-C/ Bcl-2 expression》 作者:Yunzhao Chen, Dandan Wang, Hao Peng, Xi Chen, Xueping Han, Jie Yu, Wenjie Wang, Lirong Liang, Zheng Liu, Yi Zheng, Jianming Hu, Lan Yang, Jun Li, Hong Zhou, Xiaobin Cui and Feng Li 期刊:Molecular Cancer 影響因子:7.776 PMID:30609930

《Pilose Antler Extracts (PAEs) Protect against Neurodegeneration in 6-OHDA-Induced Parkinson’s Disease Rat Models》 作者:Chaohua Li,1,2 Yanan Sun,2 Weifeng Yang,2 Shuhua Ma,2 Lili Zhang,3 Jing Zhao,4 Xin Zhao,4 and Yi Wang 期刊:Evidence-Based Complementary and Alternative Medicine  影響因子:2.064 PMID:30728849

《Celastrol inhibits microglial pyroptosis and attenuates inflammatory reaction in acute spinal cord injury rats》 作者:WangyingDaiXingguoWangHonglinTengChiLiBinWangJingWang 期刊:International Immunopharmacology 影響因子:3.118 PMID:30472522

《Dynamic decreased expression and hypermethylation of secreted frizzled-related protein 1 and 4 over the course of pulmonary fibrosis in mice》 作者:JunfeiZhouaZhengYiaQiangFu 期刊:Life Sciences 影響因子:3.234 PMID:30586565

《CLOCK and BMAL1 stabilize and activate RHOA to promote F-actin formation in cancer cells》 作者:Teng-jiao Ma, Zhi-wei Zhang, Yi-lu Lu, Ying-ying Zhang, Da-chang Tao, Yun-qiang Liu & Yong-xin Ma 期刊:Experimental & molecular Medicine 影響因子:5.584 PMID:30287810

《Inhibition of cyclin D1 enhances sensitivity to radiotherapy and reverses epithelial to mesenchymal transition for esophageal cancer cells》 作者:Huafang Su,Xiance Jin,Lanxiao Shen,Ya Fang,Zhenghua Fei,Xuebang Zhang,Congying Xie,Xiaolei Chen 期刊:Tumor Biology 影響因子:3.65 PMID:26561473

《The effects of urotensin II on migration and invasion are mediated by NADPH oxidase-derived reactive oxygen species through the c-Jun N-terminal kinase pathway in human hepatoma cells》 作者:Y Li, Z Shi, X Yu, P Feng, XJ Wang 期刊:Peptides 影響因子:2.851 PMID:27988353

《Orexin A affects HepG2 human hepatocellular carcinoma cells glucose metabolism via HIF-1α-dependent and -independent mechanism》 作者:Xing Wan , Yuanyuan Liu , Yuyan Zhao , Xiaoqi Sun, Dongxiao Fan, Lei Guo 期刊:PloS one 影響因子:2.766 PMID:28886081

《Establishment of an in vitro culture model of theca cells from hierarchical follicles in ducks》 作者:xiang gan, da chen, yan deng, junsong yuan, bo kang, jiamin qiu, wenqiang sun, chunchun han, jiwei hu, liang li, jiwen wang 期刊:Bioscience Reports 影響因子:2.899 PMID:28432272

《Loading icariin on titanium surfaces by phase-transited lysozyme priming and layer-by-layer self-assembly of hyaluronic acid/chitosan to improve surface osteogenesis ability》 作者:Yunjia Song,Aobo Ma,Jia Ning,Xue Zhong,Qian Zhang,Xu Zhang,Guang Hong,Ying Li,and Changyi Li 期刊:International journal of nanomedicine 影響因子:4.37 PMID:30425487

《Protein-protein interaction between ezrin and p65 in human breast cancer cells》 作者:R. Tang, F.X. Li, W.F. Shao, Q.S. Wen, X.R. Yu and J.B. Xiong 期刊:Genetics and Molecular Research. 影響因子:0.58 PMID:27420986

《Vitellogenin2: spermatozoon specificity and immunoprotection in mud crabs》 作者:Ya’nan Yang , Biqi Zheng , Chenchang Bao  , Huiyang Huang and Haihui Ye 期刊:Reproduction 影響因子:1.647 PMID:27458256

《Exogenous H2S prevents high glucose-induced damage to osteoblasts through regulation of KATP channels》 作者:Yuanyuan Liu,Jianyong Liu,Xin Li,Fantao Wang,Xin Xu,Chunling Wang 期刊:Biochimie 影響因子:0.134 PMID:28322929

《Verteporfin can Reverse the Paclitaxel Resistance Induced by YAP Over-Expression in HCT-8/T Cells without Photoactivation through Inhibiting YAP Expression》 作者:Pan W. Wang Q. Zhang Y. Zhang N. Qin J. Li W. Wang J. Wu F. Cao L. Xu G 期刊:Cellular Physiology and Biochemistry 影響因子:1.854 PMID:27383277

《Protective effects of allicin against ischemic stroke in a rat model of middle cerebral artery occlusion》 作者:Benping Zhang, Feng Li, Weijiang Zhao ,Jiebing Li, Qingsong Li, Weizhi Wang 期刊:Molecular Medicine Reports 影響因子:1.922 PMID:26045182

《Lethal concentration of perfluoroisobutylene induces acute lung injury in mice mediated via cytokine storm, oxidative stress and apoptosis》 作者:Y Zhang, L Fan, R Xi, Z Mao, D Shi, D Ding 期刊:Inhalation Toxicology 影響因子:1.819 PMID:28762854

《Coiled-coil domain containing 109B is a HIF1α-regulated gene critical for progression of human gliomas》 作者:Ran Xu, Mingzhi Han, Yangyang Xu, Xin Zhang, Chao Zhang, Di Zhang, Jianxiong Ji, Yuzhen Wei, Shuai Wang, Bin Huang, Anjing Chen, Qing Zhang, Wenjie Li, Tao Sun, Feng Wang, Xingang Li, and Jian Wang 期刊:Journal of Translational Medicine 影響因子:3.459 PMID:28754121

《Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model》 作者:Lina Liu, Guodong Lv, Conghua Ning, Ye Yang, Jun Zhu 期刊:Experimental and Therapeutic Medicine 影響因子:1.41 PMID:27284312

《Tumor suppressor microRNA-34a inhibits cell migration and invasion by targeting MMP-2/MMP-9/FNDC3B in esophageal squamous cell carcinoma》 作者:Lan Yang ,Xiaoyue Song ,Jianbo Zhu, Mei Li, Yu Ji, Fei Wu, Yunzhao Chen, Xiaobin Cui, Jianming Hu, Lianghai Wang, Yuwen Cao, Yutao Wei, Wenjie Zhang, Feng Li 期刊:International Journal of Oncology 影響因子:3.333 PMID:28534990

《Nanodiamond-conjugated transferrin as chemotherapeutic drug delivery》 作者:Zhiqin Wang,Zhimei Tian,Yu Dong,Lin Li,Lu Tian,Yingqi Li,Binsheng Yang 期刊:Diamond and Related Materials 影響因子:2.232 PMID:24998271

《Ras-association domain family 1 (RASSF1A) gene regulates progression, migration and invasion of bladder cancer》 作者:Yuhai Bao,Xuefeng Liu,Yang Liu,Si Wang,Bin Wu 期刊:Surgical Oncology 影響因子:2.136 PMID:

《Effect of metformin on human periodontal ligament stem cells cultured with polydopamine‐templated hydroxyapatite》 作者:Zun Yang  Xiang Gao  Mengjiao Zhou  Yunchun Kuang  Mingli Xiang  Jie Li  Jinlin Song 期刊:European Journal of Oral Sciences 影響因子:1.81 PMID:31020710

《A deuterohemin peptide protects a transgenic Caenorhabditis elegans model of Alzheimer’s disease by inhibiting Aβ1–42 aggregation》 作者:Jia Xu,Ye Yuan,Ruining Zhang,Yanhui Song,Tianzhuo Sui,Jiaqi Wang,Chonghan Wang,Yujia Chen,Shuwen Guan,Liping Wang 期刊:Bioorganic Chemistry 影響因子:3.926 PMID:30428413

《Effects of Agriophyllum squarrosum extracts on glucose metabolism in KKAy mice and the associated underlying mechanisms》 作者:Saqier,Shuyin Bao,Shuying Han,Wuli jiAo 期刊:Journal of Ethnopharmacology 影響因子:3.414 PMID:31158442

《Autologous decellularized extracellular matrix protects against H2O2-induced senescence and aging in adipose-derived stem cells and stimulates proliferation in vitro》 作者:Xiaofang Yu, Yucang He, Zhuojie Chen, Yao Qian, Jingping Wang, Ziwan Ji, Xiaoyan Tan, Liqun Li, Ming Lin 期刊:Bioscience  Reports 影響因子:2.535 PMID:31048361

《A case study of the electrical properties of astrocytes by multimode AFM》 作者:WEIDONG ZHAO,  LING‐ZHI CHEONG,  WEI CUI , SHUJUN XU , CAI SHEN 期刊:Journal of Microscopy 影響因子:2.331 PMID:31074501

《Generation of bioartificial hearts using decellularized scaffolds and mixed cells》 作者:Cailing Tong, Cheng Li, Baiyi Xie, Minghui Li, Xianguo Li, Zhongquan QiEmail author and Junjie Xia 期刊:BioMedical Engineering OnLine 影響因子:2.013 PMID:31164131

《Icariin-Functionalized Coating on TiO2 Nanotubes Surface to Improve Osteoblast Activity In Vitro and Osteogenesis Ability In Vivo》 作者:Aobo Ma, Haiyan Shang, Yunjia Song, Bo Chen, Yapeng You, Wen Han, Xu Zhang, Wenyi Zhang, Ying Li and Changyi Li 期刊:Coatings 影響因子:2.33 PMID:

《Immunogenicity analysis of decellularized cardiac scaffolds after transplantation into rats》 作者:Cailing Tong, Junjie Xia, Baiyi Xie, Minghui Li, Feifei Du, Cheng Li, Yaguang Li, Zhonggui Shan & Zhongquan Qi 期刊:Future Medicine 影響因子:2.383 PMID:31070505

《A novel STAT3 inhibitor, HJC0152, exerts potent antitumor activity in glioblastoma》 作者:Zhaoqing Li,Tingting Zhu,Yini Xu, Chuanqiang Wu,Jinliang Chen, Yu Ren,Lingping Kong, Shanshan Sun, Wenyu Guo, Yu Wang, Chao Jing, Jiabin Dong,Jia Zhou,Lun Zhang, Qiang Shen, and Xuan Zhou 期刊:American Journal of Cancer Research 影響因子:4.737 PMID:31105997

《CircSETD3 (Hsa_circ_0000567) acts as a sponge for microRNA-421 inhibiting hepatocellular carcinoma growth》 作者:Liangliang Xu, Xinfu Feng, Xiangyong Hao, Peng Wang, Yanfang Zhang, Xiaobo Zheng, Lian Li, Shengsheng Ren, Ming Zhang and Mingqing Xu 期刊:Journal of Experimental & Clinical Cancer Research 影響因子:5.646 PMID:30795787

《l-Proline Alleviates Kidney Injury Caused by AFB1 and AFM1 through Regulating Excessive Apoptosis of Kidney Cells》 作者:Huiying Li , Songli Li, Huaigu Yang ,Yizhen Wang , Jiaqi Wang , and Nan Zheng 期刊:Toxins 影響因子:3.895 PMID:30995739

《Overexpression of microRNA‐590‐3p promotes the proliferation of and inhibits the apoptosis of myocardial cells through inhibition of the NF‐κB signaling pathway by binding to RIPK1》 作者:Can Zhao , Jing Jiang , Yong‐Liang Wang , Yong‐Quan Wu 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:30321469

《Interleukin-4 signalling pathway underlies the anxiolytic effect induced by 3-deoxyadenosine》 作者:Tangxin Gao,Bai Li,Yangyang Hou,Shaolei Luo,Lei Feng,Jun Nie,Yi Ma,Le Xiao,Xu Chen,Hongkun Bao,Xianmin Lu,Feilong Huang,Gang Wang,Chunjie Xiao,Jing Du 期刊:Psychopharmacology 影響因子:3.424 PMID:30963194


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